TGF-β/SMAD Signaling Pathway Luminex Assay (CAT#: STEM-MB-0330-WXH)

Introduction

Smad (abbreviated from Sma in nematodes and Mad in Drosophila) protein is a signal transduction molecule downstream of TGF-β family receptors. There are at least 9 smad proteins currently found in vertebrates, namely：
(a) Receptor-regulated Smads (R-Smads): Smad 1, Smad 2, Smad 3, Smad 5, and Smad 8;
(b) Co-regulate Smads: Smad 4 and Smad 10;
(C) Inhibitory Smads (I-Smads): Smad 6 and Smad 7.

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Principle Applications Procedure Materials Notes Related Products

Principle

The Smad signaling pathway is the main conduction pathway of TGF-B, and its transduction process requires precise regulation of its components. Smads protein is an important signal molecule for TGF-B ligand transduction from the receptor to the nucleus to perform fibrogenic function. It mainly includes: receptor-activated Smad (receptor-activated Smad, R-Smad), such as Smad2 and Smad3; general Type Smad (common Smad, Co-Smad), such as Smad4; inhibitory Smad (inhibitorySmad, I-Smad), such as Smad6 and Smad79-0. In addition, factors that inhibit the TGFB signal transduction pathway include transcriptional co-repressor c-Ski and its related protein SnoN located in the nucleus.

Applications

To study the regulation mechanism of SMAD signal pathway in disease
To study the effect of each virus on SMAD signaling pathway
To study the effects of drugs or therapies on SMAD signaling pathways

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: AKT, BID, IAPs, tBID, SODD, Bad, MLC, Bax, PIDD, MYD88, Rac1, TLR4, PAK, RIP1, Par6, FADD, APAF-1, MKK3, ROCK, IRF7, Erk2, NFκB, RIG-1, TRAF3, LIMK, IRF9, MEKK1, p38, RIP1, TRAF5, mTOR, IRS1, MEK6, p50, SLP76, TRAM, FTO, IRS2, MSK1, p65

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