Exosomes Pathway Assay (CAT#: STEM-MB-0323-WXH)

The communication between cells mediated by exosomes is mainly through the following three methods:
1. The first is that exosomal membrane proteins can bind to target cell membrane proteins to activate signal pathways in target cells.
2. The second is that in the extracellular matrix, exosomal membrane proteins can be cleaved by proteases, and the cleaved fragments can be used as ligands to bind to receptors on the cell membrane, thereby activating intracellular signaling pathways.
3. The third is that the exosomal membrane can directly fuse with the target cell membrane, non-selectively releasing the protein, mRNA and microRNA contained in it.

To study the regulation mechanism of exosomes signal pathway in disease
To study the effect of each virus on exosomes signaling pathway
To study the effects of drugs or therapies on exosomes signaling pathways

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: RTK, PI3K, TYK2, STAT1, STAT2, STAT3, STAT5, IRAK4, JNK, MYD88, Rac1, TLR4, CREB, IRF3, MAPK, NAP1, Rel, TLR9, TCR, icam1, Mda-5, NFκB, RIG-1, TRAF3, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, CD3, IRF5, MEK3, p38MAPK, SH2, TRAF6, MHC-II, IRS1, MEK6, p50, SLP76, TRAM, PSGL1, IRS2, MSK1, TLR3, Tak1, TRIF, TNFR, ISGF3, MSK2, PI3K, TBK1, Vav, Fas, ISRE, mTOR, PKR