Multiple reaction monitoring (MRM) to detect protein in complex sample (CAT#: STEM-MB-0114-WXH)

Introduction

Multiple reaction monitoring (MRM) , also known as selective reaction monitoring (SRM), is a liquid chromatography-tandem mass spectrometry platform based on a triple quadrupole mass spectrometer, with high sensitivity, high specificity and high flux advantage. MRM analysis can quickly, sensitively and specifically quantitatively detect target analytes in highly complex protein samples, and is widely used in the quantitative analysis of various protein therapeutic molecules in the pharmaceutical industry.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Detection of apparent levels of target peptides in complex samples such as tryptic digests of human plasma
• Quantification of post-translational modifications in proteins
• Absolute Quantitative Research on Biological Disease Targets

Procedure

1. Experimental Design
2. Sample collection and processing
3. Metabolite Extraction
4. Metabolite Detection
5. Data preprocessing
6. Data analysis

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