Real-Time Quantification of Colloidal Emulsion Nucleic Acid Amplification Using Mie Scatter (CAT#: STEM-ST-0085-YJL)

Introduction

Nucleic acid amplification techniques (NAATs) are the gold standard methods for the identification of specific genomic sequences. NAATs, including polymerase chain reaction (PCR), ligase chain reaction, transcription mediated amplification, nucleic acid sequence-based amplification, are highly specific and sensitive to both RNA and DNA templates. Isothermal amplification techniques have become particularly important in the development of rapid and cost-effective diagnostics, since they require only a single temperature.
Emulsion PCR represents a particularly simple means for combining sample miniaturization with compartmentalization. Formation of >106 individual W/O colliodal emulsion droplets promotes isolation of single target templates, thus decreasing the likelihood of non-specific amplification, while improving assay accuracy and its limit-of-detection.

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Principle Applications Procedure Materials Notes Related Products

Principle

Mie scattering is defined as the type of scattering in which the diameter of the particle is the same or more than the wavelength of the radiation. Mie scattering gives a generalized solution for a system where a scattering of light takes place by a homogenous spherical medium. And this medium should have a refractive index different from that of the medium through which the light is traversing.
Unlike Rayleigh scattering, Mie scattering is not a physically independent phenomenon rather, it is a solution to Maxwell's equations for situations where the phase of the incident angle can change within the dimension of the scattering particles. Mie scattering is more commonly known as Mie solution, and it is named after Gustav Mie, a German physicist.
Mie scattering is also known as aerosol particle scattering, takes place in the atmosphere below 1,500 feet. In Mie scattering, the diameter of the spherical particles through which the light is scattered is approximately equal to the wavelength.

Applications

Mie scattering occurs in a variety of applications, including atmospheric science, cancer detection and treatment, metamaterials, and parasitology. Another application is the characterization of particles by optical scattering measurements.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Mie scattering measurement system

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