Recombinant Human B7-H4 Protein, Seafrom (CAT#: STEM-CT-0127-ZJF)

The Recombinant Human B7-H4 Protein manufactured by Seafrom is suitable for immune checkpoint in cancer therapy.
Human B7-H4, also known as V-Set Domain T Cell Activation Inhibitory Protein 1 (VTCN1), is a member of the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is upregulated by IL6 and IL10 and inhibited by GM-CSF and IL4 in antigen-presenting cells (APCs). VTCN1/B7-H4 negatively regulates T cell-mediated immune responses by inhibiting T cell activation, proliferation, cytokine production, and cytotoxicity. VTCN1 is involved in promoting epithelial cell transformation.
Storage Information:
The lyophilized (freeze-dried) form can be stored at 4°C for 24 months, while the reconstituted liquid can be stored at -20°C for 6-12 months, with a recommendation to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Protein Sequence: The DNA sequence encodes human B7-H4 (UniProtKB/Swiss-Prot: Q01973), expressed with an FC tag at the C-terminus.
Molecular Weight: Recombinant human B7-H4 protein contains 461 amino acids, with a predicted theoretical molecular weight of 52.6 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized human B7-H4 (Fc tag) at 1 μg/mL (100 μL/well) can bind to monoclonal antibodies against human B7-H4 in a linear range of 0.1-10 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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