Recombinant Human TGF-β1 Protein, Seafrom (CAT#: STEM-CT-0032-ZJF)

The Recombinant Human TGF-β1 Protein manufactured by Seafrom is suitable for cell growth, differentiation, and immune regulation.
Transforming Growth Factor-beta (TGF-β), its isoforms TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation, motility, and extracellular matrix synthesis and deposition. They play a role in various physiological processes, including embryogenesis, tissue remodeling, and wound healing. These isoforms are primarily secreted as latent complexes, stored at the cell surface and in the extracellular matrix. The release of bioactive TGF-β isoforms from latent complexes involves conformational changes induced by proteolysis, complex processing, and/or thrombospondin-1. Among these isoforms, TGF-β1 is the most abundantly secreted by nearly all cell types. Initially recognized for its ability to induce fibroblast phenotype conversion, it has more recently been implicated in the formation of skin tumors.
The lyophilized product can be stored at 2-8°C for 24 months. For reconstitution, you may use injectable water, saline, culture medium, or PBS. After reconstitution, divide it into smaller aliquots, store at -20°C for 6 months, or at -80°C for 12 months. The diluted concentration should not be lower than 100 μg/mL. Avoid repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: CED, DPD1, LAP, TGF-beta1, TGFB, TGFbeta
Protein Sequence: DNA sequence encodes human TGF-β1 (NP_000651.3) with an Fc tag at the C-terminus, predicted molecular weight of 42.2 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <0.1 EU/μg
Biological Activity: Determined by inhibiting the proliferation of mouse HT-2 cells dependent on IL-4, ED50 ≤ 0.5 ng/mL
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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