small RNA sequencing (CAT#: STEM-MB-0069-WXH)

Introduction

Small noncoding RNAs act in gene silencing and post-transcriptional regulation of gene expression. Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species). Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.
Small RNAs, including microRNAs, siRNAs, and piRNAs, are non-coding RNAs. Small RNA sequencing generally collects 18-40nt fragments in samples for sequencing analysis, studies all known small RNAs in a specific tissue in a specific state, and can also discover new small RNAs and predict their target genes.

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Applications Procedure Notes

Applications

• Understand how post-transcriptional regulation contributes to phenotype
• Identify novel biomarkers
• Capture the complete range of small RNA and miRNA species
• Cell Differentiation and Development Studies
• Regulatory Mechanism Research
• Disease marker search
• Disease Molecular Diagnosis
• Gene drug development

Procedure

1.Purification
2.Library Preparation and Amplification
3.Sequencing
4.Data analysis and storage

Notes

Customers provide total RNA, cells, or tissue samples

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