Spectral karyotyping of oocytes (CAT#: STEM-ST-0376-WXH)

Introduction

A developing egg is called an oocyte. Its differentiation into a mature egg (or ovum) involves a series of changes whose timing is geared to the steps of meiosis in which the germ cells go through their two final, highly specialized divisions.

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Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

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