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Study of High-Sensitivity and High-Specificity Biomechanical Imaging by Stimulated Brillouin Scattering (CAT#: STEM-ST-0117-YJL)

Introduction

Label-free biomechanical imaging approaches include a variety of techniques, such as atomic-force microscopy and optical coherence elastography , that probe biomechanics at high spatial resolution in complementary regimes, but require application of an external force on the sample. Second-harmonic generation microscopy circumvents this necessity, yet its applicability is limited to a small number of structural proteins. An emerging technique, spontaneous Brillouin scattering microscopy4–15, enables label-free and non-contact biomechanical imaging in three dimensions by measuring the so-called Brillouin shift (ΩB) and linewidth (ΓB), which correspond to the frequency shift and linewidth of light that is backscattered inelastically from gigahertz-frequency longitudinal acoustic phonons that are characteristic of the mechanical components of the material.




Principle

From a quantum point of view, Brillouin scattering is an interaction of light photons with acoustic or vibrational quanta (phonons), with magnetic spin waves (magnons), or with other low frequency quasiparticles interacting with light. The interaction consists of an inelastic scattering process in which a phonon or magnon is either created (Stokes process) or annihilated (anti-Stokes process). The energy of the scattered light is slightly changed, that is decreased for a Stokes process and increased for an anti-Stokes process. This shift, known as the Brillouin shift, is equal to the energy of the interacting phonon and magnon and thus Brillouin scattering can be used to measure phonon and magnon energies.

Applications

Brillouin scattering is used to determine acoustic velocities and elastic properties of a number of crystalline solids, glasses, and liquids.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Brillouin scattering measurement system (Brillouin spectrometer)
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