Study of Interaction of Hyaluronic Acid with Ethyl Violet Dye by Resonance Rayleigh Scattering (CAT#: STEM-ST-0061-YJL)

Introduction

Hyaluronic acid (HA), also known as hyaluronate or hyaluronan, is a glycosaminoglycan—a polydisperse polysaccharide with high molecular weight and an unbranched backbone composed of alternating sequences of β-(1-4)-glucoronic acid and β-(1-3)-N-acetylglucosamine moieties. It plays an important role in tissue hydration, lubrication and cellular function, and is able to hold more water than any other natural substance. Its unmatched hydrating properties result in increased smoothness, softening and decreased wrinkles. As HA is present in every tissue of the body, its importance cannot be underestimated.
Ethyl violet is an organic chloride salt that is the monochloride salt of ethyl violet cation. Used for inclusion in mixed dye solutions of the iron resorcin fuchsin type for demonstrating elastic fibres. It has a role as a histological dye and a fluorochrome. It contains an ethyl violet cation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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