T-banding (CAT#: STEM-GT-0014-WXH)

Introduction

The T-banding is a modified reverse chromosomal banding technique wherein the telomeres are strongly stained as the faint R-banding still occurs over the rest of the chromosome.

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Principle Applications Procedure Materials Notes Related Products

Applications

To study the telomeric region of chromosomes.
Stains only the end of chromosomes.
Helps preliminarily the end replication process.
Helps in studying other abnormalities associated with telomeres.

Procedure

1. Prepare slides from a good harvested cell suspension and age it for a week.
2. Air-dry it and place slides in PBS solution for 20 to 60 minutes at 87°C temperature.
3. Wash slides in PBS.
4. Stain slides in 3% Giemsa solution or acridine orange dye for 5 to 20 minutes.
5. Stain slides in Hoechst 33258 for few minutes.
6. Air dry to slides and observe under the microscope.

Materials

Microscope, coverslip and slides, PBS solution, Giemsa stain, Hoechst 33258, UV lamp and acridine orange dye.

Other recommended products

R-banding (CAT#: STEM-GT-0013-WXH)
Digital karyotyping (CAT#: STEM-GT-0020-WXH)
array Single Nucleotide Polymorphism (CAT#: STEM-GT-0018-WXH)
G-banding (CAT#: STEM-GT-0010-WXH)
Chromosomal microarray analysis (CMA) (CAT#: STEM-GT-0019-WXH)
NOR banding (CAT#: STEM-GT-0015-WXH)
Comparative Genomic Hybridisation (CGH) (CAT#: STEM-GT-0016-WXH)
array Comparative Genomic Hybridisation (aCGH) (CAT#: STEM-GT-0017-WXH)