The Detection of Indigo Carmine in Soft Drink by Resonance Rayleigh Scattering (CAT#: STEM-ST-0046-YJL)

Introduction

Food colorings can be divided into two groups: natural pigments and synthetic pigments. Synthetic pigments are widely used in food processing because they are bright in color, have good tint strength and are inexpensive. In recent years, the toxicity of synthetic food additives has been investigated.
In food production, manufacturers often use additives to improve the quality of food and its color, aroma, taste and shape. However, excessive use of food additives may have a negative impact on the health of consumers. All countries have clear and strict stipulations for the use of additives in food. Therefore, a quick method for the analysis of food additives is important for food safety. Indigo carmine (IC) is a type of food additive used in China, where the maximum allowable amount in drinks is 0.1 g/kg according to GB27600-2011 of the Ministry of Health of the People's Republic of China.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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