The Study of Interaction of Anthracycline Antineoplastic Antibiotics with Some Anionic Surfactants and Their Analytical Applications by Resonance Rayleigh Scattering (CAT#: STEM-ST-0018-YJL)

Introduction

Epirubicin (EPI), daunorubicin (DNR) and mitoxantrone (MXT), are mainly used in the treatment of acute or chronic leukemia, malignant lymphoma and other malignant tumor, belong to a group of important anthracycline anticancer antibiotics. Their effect is exerted by their inhibition of DNA and RNA synthesis, when they intercalate into the double helix of DNA and combine with the base pairs. Among them, NNR is the first generation anthracycline antibiotic, and its main defect lies on serious cardiac toxicity and bone marrow toxicity. EPI and MXT are the second generation anthracycline antibiotics, which are used more widely due to their higher efficacy and lower side effect. Especially, MXT is one of important drugs to cure breast cancer because of its outstanding activity and much lower cardiac toxicity. Therefore, it is important to determine quantificationally anthracycline antibiotics for both drug analysis and clinical assay.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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