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TNF Signaling Pathway Assay (CAT#: STEM-MB-0342-WXH)

Introduction

Tumor necrosis factor (TNF) is a cytokine produced naturally by macrophages in response to bacterial infection or other immune sources. Mainly produced by activated monocytes/macrophages, it can kill and inhibit tumor cells, promote neutrophil phagocytosis, fight infection, cause fever, induce acute protein synthesis in hepatocytes, and promote myeloid leukemia cells to differentiate into macrophages It promotes cell proliferation and differentiation, is an important inflammatory factor, and participates in the pathological damage of certain autoimmune diseases. Tumor necrosis factor (TNF) has a wide range of biological activities, and plays an important role in endotoxin shock, moxibustion, immune regulation, cell proliferation, cytotoxic activity and antiviral activity.




Principle

TNF first binds to the extracellular domain of TNF-R1 to cause trimerization of TNF-R1. Trimeric TNF-R1 recruits downstream signaling proteins such as TRADD, FADD, TRAFZ, and RIP through its aggregated intracellular death domain to assemble into a huge TNF-R1 signaling complex. This complex activates the downstream components. Signal transduction pathway, and finally activate the transcription factor NF-KB, JNK kinase, and induce cell apoptosis. Tumor necrosis factor (TNF), as a type of cytokine that can directly cause tumor cell death, works in concert with interferon to kill tumor cells.

Applications

To study the effect of each virus on TNF cell signaling pathway
To study the regulation mechanism of TNF cell signal pathway in disease
To study the effects of drugs or therapies on TNF cell signaling pathways

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: JAK1, MAPK, TYK2, STAT1, STAT2, STAT3, STAT5, IRAK4, JNK, MYD88, Rac1, TLR4, CREB, IRF3, p50, NAP1, Rel, TLR9, FADD, IRF7, Mda-5, NFκB, p65, TRAF3, GAS, IRF9, MEKK1, p38, RIP1, Vav, IRAK1, IRF5, MEK3, p38MAPK, PKR, TRAF6, IPS-1, IRS1, MEK6, PI3K, Tak1, TRAM,

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