IL-7 Detection (CAT#: STEM-MB-0276-WXH)

The biological effect of IL-7 is mainly achieved through the binding of IL-7 to the IL-7 receptor (IL-7R). IL-7R is a heterodimer composed of IL-7Rα (CD127) and a common γ chain (CD132, IL-2RG). The common γ chain is shared by multiple receptors. IL-17 mainly relies on JAK / STAT5 signaling pathway to maintain the survival status of T cells by regulating the expression of BCL-2 protein family. After IL-17 first binds to IL-17R, it triggers the activation of IL-17R-associated tyrosine kinase 1 (JAK1) and JAK3 in the cell. The activated JAK protein phosphorylates a specific motif located on the IL-17Rα chain to form a binding site, which can be used for the signaling molecule STAT5 containing the Src homolog 2 (SH2) domain to bind to phosphorylated STAT5, causing STAT5 to form dimerization The body enters the nucleus and regulates a series of genes for cell growth and survival in the nucleus, including up-regulating anti-apoptotic proteins in the BCL-2 protein family, down-regulating pro-apoptotic proteins, and promoting T cell survival in vivo.

IL-7 plays an important role in the development, proliferation, differentiation and immune self-stabilization of T cells.
IL-7 plays a role in the development and maturation of B cells and the prevention of thymus aging.
IL-7 is closely related to the occurrence of viral infectious diseases, asthma and autoimmune diseases, tumors and immune aging.

1. Process samples.
2. IL-7 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cell culture medium, tissue homogenate, etc.