Amino Acid Analysis (AAA) to determine protein quantities precisely (CAT#: STEM-MB-0115-WXH)

Introduction

If an exact knowledge of protein/peptide quantities is required for further applications, quantitative amino acid analysis, qAAA, is a suitable assay, which can not only determine protein quantities precisely, but also provide detailed information regarding the relative amino acid composition and free amino acids.

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Principle Applications Procedure Materials Notes Related Products

Principle

The most common analytical method to quantify amino acids is based on ion exchange chromatography using post-column derivatization with ninhydrin and spectrophotometric detection. Newer methodologies are based on liquid chromatographic separation with detection by mass spectrometry or spectrophotometry.

Applications

Determine protein quantities precisely

Procedure

1. Hydrolysis
2. Separation
3. Detection
4. Quantification

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