Analysis of Alcohol Dehydrogenase (ADH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1460-LGZ)

Introduction

Sensitivity: 0.29 U/L
Detection range: 0.29-248 U/L
Precision: inter-lot difference of 4.5%, intra-lot difference of 4.2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 440-460 nm

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Principle Applications Procedure Materials

Principle

Alcohol dehydrogenase catalyzes the oxidative dehydrogenation of ethanol, and NAD+ is reduced to generate NADH, which makes WST-8 orange color under the action of hydrogen-transmitting substances. The activity of alcohol dehydrogenase can be measured by measuring the change of absorbance value at 450 nm.

Applications

It is suitable for detecting the activity of alcohol dehydrogenase (ADH) in serum (plasma), animal tissue and other samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal tissue and other samples

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