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Analysis of Cathepsin L2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0080-LGZ)

Introduction

Cathepsins are a class of proteases found in the cells of various animal tissues (especially the lysosome), and are the main members of the cysteine protease family. More than 20 species have been found in the biological world, and 11 mainly exist in the human body. They are closely related to many major diseases such as human tumors, osteoporosis, and arthritis, and are a class of target proteases that have attracted much attention in recent years.
Cathepsin L2, (EC 3.4.22.43, also known as cathepsin V or cathepsin U), is a protein encoded in humans by the CTSV gene. The protein is a human cysteine cathepsin, a lysosomal cysteine protease with endopeptidase activity.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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