Analysis of CHOP (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0104-LGZ)

Introduction

GADD153 /CHOP (growth arrest and DNA-damage-inducible gene 153 or C/EBP-homologous protein) is a member of the C/EBP transcription factor family. CHOP is a marker gene of ER stress-induced apoptosis, which is involved in ER stress-regulated apoptosis and cells with defective CHOP gene expression significantly reduce ER stress-induced cell death.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma

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