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Analysis of COX7A2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1716-LGZ)

Introduction

Official Full Name: cytochrome c oxidase subunit 7A2 like<br />Also known as: EB1; SCAF1; SCAFI; SIG81; COX7AR; COX7RP<br />Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the transfer of electrons from reduced cytochrome c to oxygen. This component is a heterogeneous complex composed of three catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. Mitochondrial-encoded subunits function in electron transport and nuclear-encoded subunits may function in the regulation and assembly of the complex. The protein encoded by the nuclear gene is similar to polypeptide 1 and polypeptide 2 of the VIIa subunit of the c-terminal region, and is also highly similar to the mouse Sig81 protein sequence. The gene is expressed in all tissues and is upregulated in breast cancer cell lines after estrogen treatment. This gene likely represents a regulatory subunit of COX and mediates higher levels of energy production in target cells through estrogen. Several transcript variants of this gene have been found, some protein-coding and others non-protein-coding.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements