Analysis of H2AC18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1027-LGZ)

Introduction

Official Full Name: H2A clustered histone 18
Also known as: H2A; H2A.2; H2A/O; H2A/q; H2AFO; H2AC19; H2a-615; HIST2H2AA; HIST2H2AA3
Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, and about 146 bp of DNA are packaged in repeating units called nucleosomes. Linker histone H1 interacts with linker DNA between nucleosomes and plays a role in the compaction of chromatin into higher-order structures This gene is intronless and encodes a replication-dependent histone that is a member of the H2A histone family. The transcript of this gene lacks the poly-A tail but contains a palindromic termination element. This gene is present in a histone cluster on chromosome 1. This gene is one of four duplicated histone genes in the cluster; this record represents the centromeric copy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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