Analysis of ICOS (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0263-LGZ)
Introduction
ICOS is an immune checkpoint expressed on activated T cells, a type of co-stimulatory receptor, which can induce the proliferation, survival and differentiation of T cells, and regulate various T cell functions.
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Immunology/Inflammation
Procedure
1. Coat the capture antibody on the well plate. 2. Add samples/calibrators. 3. Add detection antibody. 4. Read the plate and analyze the data.