Analysis of IL6R Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1067-LGZ)

Introduction

Official Full Name: interleukin 6 receptor
Also known as: IL6Q; gp80; CD126; HIES5; IL-6R; IL6RA; IL6RQ; IL-1Ra; IL-6RA; IL6QTL; IL-6R-1
This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and the interleukin 6 signal converter (IL6ST/GP130/IL6-β), a receptor subunit shared by many other cytokines. Dysregulation of IL-6 and its receptors has been implicated in the pathogenesis of various diseases such as multiple myeloma, autoimmune diseases and prostate cancer. Alternative splicing transcript variants encoding different isoforms have been identified in this gene. A pseudogene of this gene has been found on chromosome 9.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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