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Analysis of Influenza A NP (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0367-LGZ)

Introduction

The nucleoprotein (NP) of influenza A virus wraps the negative strand of viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. While binding to ssRNA, NP is able to self-associate to form large oligomeric complexes. NP is capable of interacting with a variety of viral and other macromolecules of cellular origin.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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