Analysis of KIF1B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0076-LGZ)

Introduction

Also known as: KLP; CMT2; CMT2A; CMT2A1; HMSNII; NBLST1<br />Expected to enable microtubule binding activity and plus-end oriented microtubule motility activity. Predicted to be involved in chemical synaptic transmission; dense nucleosome cytoskeletal transport; and vesicle-mediated transport. Expected to act upstream or within mitochondrial transport along microtubules. Predicted to be located in cytoplasmic vesicle membranes and neuronal processes. Expected to be part of the dynein complex. Predicted to be part of kinesin complex. Predicted to be active in several cellular components, including axon; dendrite; and microtubule. Implicated in Charcot-Marie-Tooth disease type 2A1; pheochromocytoma. Biomarker of hepatocellular carcinoma.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Charcot-Marie-Tooth disease type 2, Neuroblastoma, Pheochromocytoma

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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