Analysis of Pepsinogen (PGN) by Raman Spectroscopy (CAT#: STEM-ST-0012-WXH)

Introduction

Pepsinogen (PGN) is released by the glandular cells (chief cells) in the stomach. PGN occurs in several molecular forms classified in two groups (with different histochemical properties): PGNs I (observed in the main cells and right mucous glands of stomach) and PGNs II (seen also in the antrum glands). Conversion of PGN to PEP is stimulated by low pH (the presence of hydrochloric acid) and autocatalytic cleavage of the peptide blocking the active center of the enzyme.

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Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

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