Analysis of Total Sulfhydryl Group/Total Thiol (-SH) by Enzyme-labeled Instrument (CAT#: STEM-MB-1424-LGZ)

Introduction

Sensitivity: 9.91 μmol/L
Detection range: 9.91-1000 μmol/L
Precision: inter-lot difference of 2.9%, intra-lot difference of 2.5%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 410-420 nm

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Principle Applications Procedure Materials

Principle

The sulfhydryl compound reacts with 5,5' -dithiobide (2-nitrobenzoic acid) to produce yellow products under neutral or alkaline conditions, and has the maximum absorption peak at 412 nm.

Applications

Used for the determination of total sulfhydryl in serum, plasma and animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma and animal tissue samples

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