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Antibody Microarray (CAT#: STEM-MB-0149-WXH)

Introduction

An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a collection of captured antibodies are spotted and fixed on a solid surface such as glass, plastic, membrane, or silicon chip, and the interaction between the antibody and its target antigen is detected. Antibody microarrays are often used for detecting protein expression from various biofluids including serum, plasma and cell or tissue lysates. Antibody arrays may be used for both basic research and medical and diagnostic applications.




Applications

• Detection and study of protein abundance, function, pathways, and potential drug targets
• Signalling Pathway Related Research
• Drug Mechanism Research
• Clinical Research of autoimmune diseases, infectious diseases, cancer, neurodegenerative diseases

Procedure

1. Protein sample preparation(cells, tissues, serum or plasmid)
2. Proteins are labeled with biotin.
3. Biotinylated samples are incubated with antibody array.
4. Detection by dye conjugated streptavidin.

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