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Protein Microaray (CAT#: STEM-MB-0143-WXH)

Introduction

A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel. The chip consists of a support surface such as a glass slide, nitrocellulose membrane, bead, or microtitre plate, to which an array of capture proteins is bound. Probe molecules, typically labeled with a fluorescent dye, are added to the array. Any reaction between the probe and the immobilised protein emits a fluorescent signal that is read by a laser scanner. Protein microarrays are rapid, automated, economical, and highly sensitive, consuming small quantities of samples and reagents.




Applications

• Protein expression profiling
• Protein functional analysis: identification of protein–protein interactions, protein–phospholipid interactions, small molecule targets, enzymatic substrates and receptor ligands.
• Characterization of antibody
• Treatment development
• Detection of antigens and antibodies in blood samples
• Profiling of sera to discover new disease biomarkers
• Monitoring of disease states and responses to therapy in personalized medicine
• Monitoring of environment and food.

Procedure

Preparation of Protein microaray
1. Selection and handling of substrate material
2. Immobilizing proteins onto the substrate
Detection of Protein microaray
1. Extraction and purification of protein
2. Labeling of protein
3. Hybridization with protein chip
4. Scanning and analysis of results

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