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DNA Microarray (CAT#: STEM-MB-0142-WXH)

Introduction

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target.




Applications

• Analysis of transcriptomes and proteomes
• Diagnose several pathogenic and genetic diseases in man.
• Identify microbes in the environment.
• Genotyping of genomes through single nucleotide polymorphism(SNP)analysis.
• Detect gene expression by analyzing cDNAs produced from mRNAs of a cell type at different times.
• Measure changes in gene expression levels
• Observe genomic gains and losses.
• Observe mutations in DNA

Procedure

1. Collect samples
2. Isolate mRNA
3. Create labelled cDNA
4. Hybridization
5. Detect the relative intensities of fluorescence under Microarray Scanner
6. Analyze data

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