Assay for Transposase Accessible Chromatin With Sequencing (ATAC-seq) (CAT#: STEM-MB-0060-WXH)

Introduction

ATAC-seq stands for Assay for Transposase Accessible Chromatin with high-throughput sequencing, which is a high-throughput sequencing technology that uses transposase to study chromatin accessibility. DNA replication and gene transcription require the high-level structure of DNA to be unwrapped, and the opened chromatin allows some regulatory proteins (such as transcription factors and cofactors) to bind to it. This characteristic of chromatin is called chromatin accessibility.
ATAC-seq is currently the mainstream tool for the study of chromatin conformation. The principle is that random transposition is carried out on the chromatin DNA in the nucleus by Tn5 transposase: the chromatin in the open area can be transposed due to DNA exposure; the chromatin in the compact area is compressed and folded due to DNA, transposition Enzymes are inaccessible, so transposition cannot occur. The successfully transposed DNA is added with adapter sequences for sequencing, and through PCR amplification, high-throughput sequencing and bioinformatics analysis, the higher the degree of chromatin opening, the stronger the sequencing signal.