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Calcium Signaling Pathway Luminex Assay (CAT#: STEM-MB-0326-WXH)

Introduction

Almost all physiological activities are regulated by Ca2+. The basis of Ca2+ as a cell messenger is that there is a concentration gradient between cytoplasmic Ca2+ and intracellular calcium store or extracellular Ca2+. When a certain stimulus greatly increases the intracellular Ca2+ concentration, it plays a role in transmitting signals. The characteristics of Ca2+ itself are more conducive to binding to the target protein, thereby transmitting information. Due to the integration of multiple functions, the Ca2+ signal must be very flexible and must be strictly controlled at the same time. It is realized by means of different intermediate links of the Ca2+ signal, that is, Ca2+ can act with different amplitudes in different time and space. Different types of cells complete their specific physiological functions with different combinations of calcium signals.




Principle

At the cellular level, the calcium signaling pathway can be divided into three levels; at first, it is the most fundamental event.It is derived from a very low level of stimulation that will cause a short opening of a single channel.The released calcium is called calcium pulse (calci-umblips) or Calcium quarks (calciumquarks); then the basic event, which originates from the opening of a small group of channels, and the released calcium forms calcium puffs or calcium scintillations (calci2umsparks). Finally, when the stimulation intensity is high, the synchronization of a large number of basic events produces an overall calcium signal.

Applications

To study the regulation mechanism of calcium signal pathway in disease
To study the effect of each virus on calcium signaling pathway
To study the effects of drugs or therapies on calcium signaling pathways

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: NCK, DAG, PIP2, IP3, STAT2, STAT3, SYK, IRAK4, JNK, MYD88, Rac1, TLR4, ROC, IRF3, MAPK, NAP1, Rel, TLR9, TCR, icam1, Mda-5, NFκB, RIG-1, TRAF3, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, CALM, IRF5, MEK3, p38MAPK, SH2, TRAF6, MHC-II, IRS1, MEK6, p50, SLP76, TRAM, PSGL1, IRS2, MSK1, TLR3, Tak1, TRIF, IKB, ISGF3, MSK2, PI3K, TBK1, Vav, PMCA, ISRE, mTOR, PKR

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