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CAMs Signaling Pathway Assay (CAT#: STEM-MB-0361-WXH)

Introduction

Cell adhesion molecules (CAMs) are a group of membrane glycoprotein and carbohydrate molecules that mediate the adhesion of cells to cells or of cells to the extracellular matrix.
Cell adhesion molecules are (glyco)proteins expressed on the cell surface and play a critical role in a wide array of biologic processes that include hemostasis, the immune response, inflammation, embryogenesis, and development of neuronal tissue. There are four main groups: the integrin family, the immunoglobulin superfamily, selectins, and cadherins.




Principle

CAM-mediated changes in neuronal behavior depend on a number of intracellular signaling cascades including changes in various second messengers, among which CAM-dependent changes in intracellular Ca2+ levels play a prominent role. Ca2+ is an essential secondary intracellular signaling molecule that regulates fundamental cellular functions in various cell types, including neurons.

Applications

To study the regulation mechanism of CAMs signal pathway in disease
To study the effect of each virus on CAMs signaling pathway
To study the effects of drugs or therapies on CAMs signaling pathways

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: JAK1, JAK2, TYK2, STAT1, STAT2, STAT3, STAT5, IRAK4, JNK, MYD88, Rac1, TLR4, CREB, IRF3, MAPK, NAP1, Rel, TLR9, FADD, IRF7, Mda-5, NFκB, RIG-1, TRAF3, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, Histone-H3, IRF5, MEK3, p38MAPK, SH2, TRAF6, IKK-α, IRS1, MEK6, p50, SLP76, TRAM, IKK-β, IRS2, MSK1, p65, Tak1, TRIF, IPS-1, ISGF3, MSK2, PI3K, TBK1, Vav, IRAK1, ISRE, mTOR, PKR, TLR3

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