Cytometric Bead Array (CBA) (CAT#: STEM-CBT-0055-WXH)

Introduction

Cytometric bead array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. Compared to other quantifier assays, as enzyme-linked immunosorbent assay (ELISA) and Western blot, CBA significantly reduces sample requirements and time to results.
This technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies.
This new technology allows for (1) evaluation of multiple analytes in a single
sample; (2) utilization of minimal sample volumes to glean data; (3) reproducibility and results comparative with previous experiments;
(4) direct comparison with existing assays; and (5) a more rapid evaluation of multiple samples in a single platform.

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Principle Applications Procedure Materials Notes Related Products

Principle

The basic idea behind the bead-based capture array technology is that each type of bead has a specific protein-capturing antibody conjugated on to its surface. Each antibody has its own specific fluorescent intensity. Sometimes, you can even sort the beads by size. In a single experiment, you incubate your sample with a variety of beads to detect a wide spectrum of proteins of interest. Based on the fluorescence intensity of the beads (or size), you can determine the specific protein that each bead captured.

Applications

• Oncology research and monitoring of adjuvant diagnosis, efficacy and prognosis of oncological diseases.
• Stem cell research and monitoring of stem cell therapy.
• Immunology research and ancillary diagnosis and monitoring of immune diseases.
• Infectious disease research and ancillary diagnosis and efficacy evaluation.
• Immune function monitoring.
• Drug efficacy evaluation, drug development, vaccine research.
• Quantitative analysis of phosphorylated proteins and apoptosis-related proteins in cell signaling.

Procedure

1. Sample & Standard Preparation
2. Incubation of the sample with capture beads
3. Labeled fluorescent detection reagent
4. Washing
5. Flow cytometry assay
6. Data analysis

Materials

FITC, PE label

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