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Genome de novo sequencing is de novo sequencing, which is to sequence a species without relying on any reference sequence, and then splicing and assembling through bioinformatics analysis methods to obtain a complete gene sequence map of the species.
The most important and critical part of de novo sequencing is to splice and assemble the small fragments that have been measured. If splicing errors occur during this process, the entire sequencing result will be inaccurate. Therefore, multiple digestion of the sample to be tested and reverse verification of the sequence before sequencing are the key factors to ensure full coverage of the fragments and accuracy of the sequencing results.