IL-28 Detection (CAT#: STEM-MB-0295-WXH)

Introduction

Interleukin-28 (IL-28A, IL-28B) is a group of new interleukins produced by a variety of cells induced by viruses or double-stranded RNA, such as peripheral blood mononuclear cells (PMBC), dendritic cells ( DC) and HeLa cells. Its genetic structure is similar to IL-10, but its amino acid level is closer to interferon (IFN). So Kotenko et al. also refer to them accordingly as IFN-λ2 (IL-28A) and IFN-λ3 (IL-28B). It can bind to a heterodimeric type II cytokine receptor composed of IL-10Rβ and IL-28Rα, and exerts its antiviral or other defense functions through the Jak-STAT signaling pathway. The newly discovered IL-28 has similar functions to class I IFN and can selectively act on different types of target cells. They can be used as a substitute for IFN for the treatment of tumors and viral diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

Experiments show that IL-28 must bind to the receptor before it can function. The IL-28 receptor is a heterodimeric type II cytokine receptor, which is composed of the IL-10 receptor β subunit (IL-10Rβ also known as IL-10R2, CRF2-4) and IL-28Rα or IFN-λR1, CRF2-12). IL-10Rβ is also a component of IL-10 and IL-22 receptors. IL-28Rα determines the specificity of binding to IL-28, and may mediate intracellular signaling. IL-28 binds to its receptor and transmits information through Jak-STAT pathway. First, after IL-28 binds to the receptor, it initiates a signaling cascade. After a multi-step phosphorylation reaction, STAT2 is activated, resulting in the interaction of IFN-stimulated gene factor 3 complex (ISGF3) and IFN-stimulated response element (ISRE), thereby regulating gene transcription.

Applications

The main functions of IL-28 include anti-virus, anti-cell proliferation and immune regulation.
IL-28 enhances the expression of MHC class I molecules in target cells, promotes cell processing and antigen presentation, and enhances antiviral specific immune responses.

Procedure

1. Process samples.
2. IL-28 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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