IL-31 Detection (CAT#: STEM-MB-0297-WXH)

Introduction

IL-31 belongs to the IL-6 cytokine family and is mainly expressed in activated Th2 cells, especially activated helper Th2 cells, mast cells, monocytes/macrophages and dendritic cells. The main targets of IL-31 are located in the skin, lungs, intestines, and nervous system. It is also expressed at low levels in tissues such as testis, bone marrow, thymus, and spleen. IL-31 plays a major role in the induction of chronic inflammation. When the tissue is exposed to the environment, IL-31 regulates the immune process of adaptive immunity and acquired immunity. In addition, it can further stimulate the secretion of pro-inflammatory cytokines and chemokines.

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Principle Applications Procedure Materials Notes Related Products

Principle

The functional receptors of IL-31 are IL-31 Receptor A (IL-31RA) and oncostatin M Receptor (OSMR), which combine to form a heterodimer complex. IL-31RA is expressed in skin tissue, brain, lung, trachea, skeletal muscle, thymus, spleen, placenta and other tissues. OSMR is more widely expressed, most in trachea, thymus and skin. In cells where OSMR and IL-31RA exist, IL-31 can activate transcription factors STAT1 and STAT3-6. But in cells with only IL-31RA or OSMR, these transcription factors did not respond to IL-31. After IL-31 binds to IL-31R, it mainly transmits cell signals through the activation of signal transducers and activators of transcription (STATs), mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways to play a biological role.

Applications

IL-31 is associated with many human diseases(atopic dermatitis, allergic asthma).
IL-31 can induce chemokine gene expression in human epidermal keratinocytes (NHEK).
IL-31 has a direct relationship with the body's own immune system against allergens and pathogens.

Procedure

1. Process samples.
2. IL-31 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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