IL-5 Detection (CAT#: STEM-MB-0274-WXH)

Mouse IL-5 is composed of 133 amino acid residues and contains a 21 amino acid signal peptide. The mature IL-5 molecule contains 112 amino acid residues, and the molecular chain is 12-15 kDa. There are 3 glycosylation sites, and the molecular weight after carbonylation is 18kDa. Glycosylation plays an important role in IL-5 activity expression and binding of corresponding receptors. Mouse IL-5 usually exists as a disulfide-linked dimer with a molecular weight of 45 kDa. Human IL-5 is composed of 134 amino acid residues, contains a signal state of 22 amino acids, and has 2 glycosylation sites. Mouse IL-5 gene is located on chromosomes 5 and 11 respectively, and is closely linked to the genes of IL-3, IL-4, and GM-CSF hematopoietic factors.

IL-5 can replace T cells to cooperate with thymus-dependent antigen antibody response in vitro.
IL-5 has important regulatory effects on the proliferation and differentiation of B cells and eosinophils.
IL-5 promotes antigen-stimulated B cells to differentiate into antibody-synthesizing cells
IL-5 promotes IgA synthesis.
IL-5 cooperates with ConA or IL-2 to induce the differentiation of killer T cell precursors (CTPp) into CTL in the thymus.

1. Process samples.
2. IL-5 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cell culture medium, tissue homogenate, etc.