Measurement of serum electrolytes by Ion Selective Electrode (CAT#: STEM-ACT-0098-WXH)

Introduction

Electrolytes play an important role in controlling acid base balance, blood clotting, and body fluid and muscle contractions. Serum electrolytes concentrations are most commonly used tests for assessment of a patient's clinical conditions, and are associated with morbidity and mortality.

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Principle Applications Procedure Materials

Principle

An ideal ion-selective electrode (I.S.E.) consists of a thin membrane across which only the intended ion can be transported. The transport of ions from a high conc. to a low one through a selective binding with some sites within the membrane creates a potential difference.

Applications

• Used for the potentiometric determination of several anions (fluoride, chloride, bromide, iodide ion) and cations (sodium, potassium, calcium ion).
• Used for endpoint detection in potentiometric titration.
• In biochemistry, ion selective electrodes are used for the determination of enzymes and substances which interact with enzymes.

Procedure

1. Preparation of Standards
2. Instrument Setup and Operation
3. Analysis of sample

Materials

Ion Selective Electrode

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