Recombinant Human PD-1 Protein (His), Seafrom (CAT#: STEM-CT-0146-ZJF)

The Recombinant Human PD-1 Protein (His) manufactured by Seafrom is suitable for immune checkpoint regulation.
Programmed cell death protein 1 (PD-1), also known as CD279 and PDCD1, is a type I membrane protein and a member of the extended CD28/CTLA-4 family of T cell regulators. PD-1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells, and a subset of thymocytes. It has two ligands, PD-L1 and PD-L2, both of which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is mainly found on dendritic cells (DCs) and a few tumor lines.
PD-1 inhibits T-cell proliferation and the production of related cytokines, including IL-1, IL-4, IL-10, and IFN-γ, by suppressing the activation and transduction of the PI3K/AKT pathway. Additionally, PD-1 inhibits B-cell receptor (BCR)-mediated signaling by dephosphorylating key signal transducers. In vitro, treatment of anti-CD3-stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
For storage, lyophilized (freeze-dried) products can be stored at 4°C for 24 months. After reconstitution, the liquid can be stored at -20°C for 6-12 months, and it is recommended to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: PDCD1, CD279, SLEB2
Protein Sequence: The DNA sequence encodes human PD-1 (NCBI Reference Sequence: NP_005009.2), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human PD-1 protein contains 148 amino acids, with a predicted theoretical molecular weight of 21.16 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized human PD-1 (His tag) at 0.5 μg/mL (100 μL/well) can bind to human PD-L1 (Fc tag) in a linear range of 0.5-10 μg/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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