Recombinant Human Siglec-3/CD33 Protein, His-Avi Tag, Yisheng (CAT#: STEM-CT-0243-ZJF)

The Recombinant Human Siglec-3/CD33 Protein, His-Avi Tag manufactured by Yisheng is suitable for studying Siglec-3/CD33-mediated cellular processes.

Source: Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Asp18-His259.[Uniprot | P20138-1]
Molecular Weight: The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin: Less than 1EU per μg by the LAL method.
Purity: > 95% as determined by Tris-Bis PAGE and HPLC
Activity: ELISA Data: Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
Formulation: Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 8.0).

Shipped with ice pack.
Stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 ℃ under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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