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Subcellular Localization to localize protein (CAT#: STEM-MB-0235-WXH)

Introduction

The interior of a cell can be further divided into different cellular regions or organelles, known as subcellular. Mature proteins must be in specific subcellular organelles to exert stable biological functions. Common subcells include nucleus, cell membrane, endoplasmic reticulum, Golgi apparatus, cytoplasm, mitochondria, chloroplast, etc. Subcellular localization is to find the specific location of biological macromolecules in the cell, such as in the nucleus, in the cytoplasm or on the cell membrane.




Applications

• Provide information to clarify gene function
• To speculate on the biological function of proteins

Procedure

1. Subcellular localization prediction software analysis
2. Plant overexpression vector construction
3. Transformation of Agrobacterium
4. Agrobacterium-mediated dipping of tobacco or rice
5. Subcellular Localization
6. Laser Confocal Scanning

Notes

Customers provide sequence information or plasmid or strain.
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