Wee1 Signaling Pathway Assay (CAT#: STEM-MB-0316-WXH)

The conduction of the wee1 signaling pathway can be described in two ways: 1). The role of Wee1 protein kinase in the cell cycle. In the G2/M phase of the cell, Wee1 protein kinase inactivates the CDK1/Cyclin B complex by inhibiting CDK1 activity by phosphorylating the Tyr15 site of CDK1, thereby inhibiting the cell cycle into the mitosis phase; in the S phase, Wee1 protein kinase that acts as the chromatin synthesis sensor has two consecutive phosphorylation events. One is to phosphorylate the Tyr15 site of CDK1 throughout the S phase of the cell, preventing cells from entering the next cell cycle phase until DNA replication or repair is complete; The second is to phosphorylate the Tyr37 site of histone H2B, stop histone synthesis, and maintain the correct histone-to-DNA ratio before entering the mitosis phase.
2). The role of Wee1 protein kinase in DNA damage response. Wee1 protein kinase phosphorylates the Tyr15 site of CDK1/CyclinB complex CDK1, which inactivates the cell cycle to prevent DNA repair in the G2 phase.

To study the regulation mechanism of wee1 signal pathway in disease
To study the effect of each virus on wee1 signaling pathway
To study the effects of drugs or therapies on wee1 signaling pathways

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Detectable targets: RTK, PI3K, TYK2, STAT1, STAT2, STAT3, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, TNFR, ISGF3, MSK2, PI3K, TBK1, Vav, MHC-II, IRS1, MEK6, p50, SLP76, TRAM, STAT5, IRAK4, JNK, MYD88, Rac1, TLR4, PSGL1, IRS2, MSK1, TLR3, Tak1, TRIF, CREB, IRF3, MAPK, NAP1, Rel, TLR9, CD3, IRF5, MEK3, p38MAPK, SH2, TRAF6, Fas, ISRE, mTOR, PKR, RIG-1, TRAF3, TCR, icam1, Mda-5, NFκB