Analysis Immunoassay of Carcinoembryonic Antigen (CEA) by KinExA (CAT#: STEM-MB-0076-CJ)

Introduction

Carcinoembryonic antigen (CEA) is an embryonic glycoprotein present in mucosal cells. Carcinoembryonic proteins are usually produced in the gastrointestinal tissue during fetal development, but are no longer produced after birth. Healthy adults usually have very low levels of carcinoembryonic proteins in their blood (about 2-4 ng/ml). However, people with some types of cancer have elevated levels of carcinoembryonic antigen in their blood. Carcinoembryonic antigen levels can be used as a marker, and heavy smokers may also have elevated levels of carcinoembryonic antigen in their blood.

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Principle Applications Procedure Materials Notes Related Products

Principle

KinExA is a two-stage analysis system. In the first stage, a number of solutions are prepared, where one partner remains constant (constant binding partner, or CBP) and the other (titrant) is variable, usually serially diluted. As the titrant is added, the free CBP decreases and is analysed by a sophisticated and precise microfluorescence measurement device. The signal generated can be mathematically related to the affinity (KD) of the two molecules for each other, as well as the kinetic parameters of binding (kon) and dissociation (koff).

Applications

Oncology & Cancer; Immunology/Inflammation; Pharmacology

Procedure

1. preparation of the functionalized beads which will capture the analyte for measuremen.
2. preparation of a series of solutions consisting of a constant initial concentration of one component of the binary reaction and serial dilutions of the other reactant. The component that is kept constant is the constant binding partner (CBP) , and is the one which will be analyzed.
3. each reaction mixture is sampled and the fluorescence of free CBP bound to the capture beads is obtained for subsequent numerical analysis.

Materials

• Sample Type: Serum, Plasma, Urine
• Equipment: Kinetic Exclusion Assay (KinExA)

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