Analysis of Glutamic Acid by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1357-LGZ)

Introduction

Sensitivity: 4.00 μmol/L
Detection range: 4.00-450 μmol/L
Precision: inter-lot difference of 2.9%, intra-lot difference of 2.2%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 340 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Glutamic Acid and NAD+ produce α-ketoglutaric acid, NADH and NH4+ under the action of glutamic acid dehydrogenase. The maximum absorption of NADH is at 340 nm. The content of glutamic acid can be calculated by measuring the change of NADH.

Applications

Used to detect glutamic acid content in serum, plasma, animal tissue, cells and cell supernatant.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, animal tissue, cells and cell supernatant

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