Analysis of Hydroxyproline (HYP) by Enzyme-labeled Instrument (CAT#: STEM-MB-1456-LGZ)

Introduction

Sensitivity: 0.04 μg/mL
Detection range: 0.04-10 μg/mL
Precision: inter-lot difference of 4.4%, intra-lot difference of 1.2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 550-570 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

The sample was hydrolyzed to produce free hydroxyproline. The oxidation product produced under the action of oxidant reacted with color developing agent to be purplish red, with absorption peak at 558 nm. Within a certain range, its absorbance and concentration showed a linear relationship. By measuring the absorbance at 558 nm, the content of hydroxyproline in the sample can be calculated.

Applications

For the determination of hydroxyproline in serum, animal tissue and urine samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, animal tissue and urine samples

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