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Analysis of IL-22Rα2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0333-LGZ)

Introduction

Interleukin-22 Receptor Subunit α-2 (IL22Rα2) belongs to the type ll cytokine receptor family. IL22Rα2 is a secreted protein and contains three fibronectin type-lll domains. IL22Rα2 is widely expressed in many tissues. IL22Rα2 functions as an IL22 antagonist and may be important in the regulation of inflammatory response. Three altemnatively spliced transcript variants encoding distinct isoforms have been described.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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