Analysis of Peroxidase (POD) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1328-LGZ)

Introduction

Sensitivity: 0.5 U/mL
Detection range: 0.5-40 U/mL
Precision: inter-lot difference of 5.5%, intra-lot difference of 3.8%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 240 nm、420 nm

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Principle Applications Procedure Materials

Principle

Peroxidase (POD) was used to catalyze the decomposition of hydrogen peroxide into water and oxygen, and oxygen oxidized pyrogallic acid to form yellow products. The enzyme activity was obtained by measuring the change of absorbance at 420 nm.

Applications

It is suitable for detecting the activity of peroxidase (POD) in plant tissue, but not for serum (serum).

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: Plant tissue

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