Analysis of Plant Flavonoids by Enzyme-labeled Instrument (CAT#: STEM-MB-1425-LGZ)

Introduction

Sensitivity: 0.66 -μg/mL
Detection range: 0.66 - 150 μg/mL
Precision: inter-lot difference of 5.3%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 500-520 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

In the alkaline nitrite solution, flavonoids and aluminum ions form red complex, determination of the sample extract at 510 nm light absorption value, can calculate the flavonoid content of the sample.

Applications

Used to detect flavonoid content in plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: plant tissue samples

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