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Cell Scratch Test to measure cell migration (CAT#: STEM-CBT-0021-WXH)

Introduction

The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro.
The principle of this method is to artificially create a blank area called a "scratch" on the fused monolayer of cells, and the cells at the edge of the scratch will gradually enter the blank area to heal the "scratch". It simulates the process of cell migration in vivo and is a relatively simple method in in vitro experiments to study cell migration. The cell scratch test is a simple and easy method to detect cell movement with low experimental cost, and can be used to detect the invasion and metastasis ability of adherent growth tumor cells.




Applications

• Research on cell migration and repair ability
• Observation of the effect of an exogenous factor on cell migration
• Studying the Metastatic Ability of Tumor Cells

Procedure

1. Draw a horizontal line evenly on the back of the orifice plate
2. Adding Cells to the Wells
3. The next day, use the tip of the gun to hang down to the horizontal line scratches on the back
4. Washing away the streaked cells
5. Cultivate at different times, take samples, and take pictures
6. Data processing

Notes

Customers provide cell lines, required drugs, drug treatment methods and concentrations.

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