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The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro.
The principle of this method is to artificially create a blank area called a "scratch" on the fused monolayer of cells, and the cells at the edge of the scratch will gradually enter the blank area to heal the "scratch". It simulates the process of cell migration in vivo and is a relatively simple method in in vitro experiments to study cell migration. The cell scratch test is a simple and easy method to detect cell movement with low experimental cost, and can be used to detect the invasion and metastasis ability of adherent growth tumor cells.